The mission of the Tennessee Wildlife Resources Agency is to preserve, conserve, protect, and enhance the fish and wildlife of the state and their habitats for the use, benefit, and enjoyment of the citizens of Tennessee and its visitors. The Agency will foster the safe use of the state's waters through a program of law enforcement, education, and access.

TWRA is divided into four regions.

TWRA Region 1 covers the following counties:
Benton, Carroll, Chester, Crockett, Decatur, Dyer, Fayette, Gibson, Hardeman, Hardin, Haywood, Henderson, Henry, Houston, Humphreys, Lake, Lauderdale, Madison, McNairy, Obion, Perry, Shelby, Stewart, Tipton, Weakley

TWRA Region 2 covers the following counties:
Bedford, Cannon, Cheatham, Coffee, Davidson, Dickson, Franklin, Giles, Hickman, Lawrence, Lewis, Lincoln, Macon, Marshall, Maury, Montgomery, Moore, Robertson, Rutherford, Smith, Sumner, Trousdale, Wayne, Williamson, Wilson

TWRA Region 3 covers the following counties:
Bledsoe, Bradley, Clay, Cumberland, DeKalb, Fentress, Grundy, Hamilton, Jackson, Marion, McMinn, Meigs, Monroe, Morgan, Overton, Pickett, Polk, Putnam, Rhea, Roane, Sequatchie, Van Buren, Warren, White

TWRA Region 4 covers the following counties:
Anderson, Blount, Campbell, Carter, Claiborne, Cocke, Grainger, Greene, Hamblen, Hancock, Hawkins, Jefferson, Johnson, Knox, Loudon, Scott, Sevier, Sullivan, Unicoi, Union, Washington