| Norfolk Southern | Main Line - Montview to Salisbury | 36.02729 | -79.86313 | 40 |
| Norfolk Southern | Main Line - Salisbury to Greenville | 35.40269 | -80.67711 | 25 |
| CSX Transportation | Aberdeen Subdivision | 35.56371 | -79.15551 | 35 |
| CSX Transportation | Andrews Subdivision | 34.81503 | -79.64352 | 5 |
| CSX Transportation | Blueridge Subdivision/Clinchfield | 35.56691 | -79.59902 | 225 |
| Norfolk Southern | CF Line | 35.79186 | -79.54003 | 24 |
| CSX Transportation | Charlotte Subdivision | 35.20992 | -80.86113 | 30 |
| Norfolk Southern | CL Line | 34.88163 | -77.38396 | 20 |
| Norfolk Southern | D Line | 36.16791 | -78.71929 | 15 |
| Norfolk Southern | DW Line | 36.48319 | -79.77217 | 10 |
| Norfolk Southern | EC Line | 35.13584 | -77.31804 | 41 |
| Norfolk Southern | H Line - Greensboro to Selma | 36.02325 | -79.31733 | 25 |
| Norfolk Southern | H Line - Selma to Goldsboro | 35.60412 | -78.3635 | 26 |
| CSX Transportation | Hamlet Subdivision | 34.84547 | -79.74789 | 5 |
| CSX Transportation | Hamlet Terminal Subdivision | 34.9158 | -79.66174 | 4 |
| Norfolk Southern | J Line | 35.93523 | -79.05612 | 3 |
| Norfolk Southern | K Line | 36.17799 | -80.18273 | 24 |
| Norfolk Southern | L Line | 35.67535 | -80.55901 | 35 |
| Norfolk Southern | M Line | 35.82348 | -79.90994 | 12 |
| CSX Transportation | Monroe Subdivison | 34.97845 | -80.39971 | 35 |
| Norfolk Southern | N Line | 35.53009 | -80.3439 | 13 |
| Norfolk Southern | NB Line | 35.32429 | -77.08458 | 15 |
| CSX Transportation | Norlina Subdivision | 36.13586 | -78.38921 | 25 |
| CSX Transportation | North End Subdivision | 36.20953 | -77.63643 | 23 |
| Norfolk Southern | NS Line | 36.03809 | -78.70831 | 20 |
| Norfolk Southern | O Line | 35.41069 | -80.84285 | 15 |
| CSX Transportation | Parmele Subdivision | 35.66795 | -77.35174 | 12 |
| CSX Transportation | Portsmouth Subdivision | 36.47661 | -77.43939 | 10 |
| Norfolk Southern | R Line | 35.15477 | -80.86737 | 6 |
| Norfolk Southern | S Line | 35.73354 | -81.5001 | 57 |
| CSX Transportation | South End Subdivision | 35.17462 | -78.4604 | 70 |
| CSX Transportation | Tarboro Subdivsion | 35.86032 | -77.29058 | 29 |
| CSX Transportation | Terrell Subdivison | 35.5055 | -81.05801 | 15 |
| Norfolk Southern | VF Line | 35.51231 | -78.8012 | 32 |
| CSX Transportation | W&W Subdivision | 35.08416 | -77.96898 | 20 |
| CSX Transportation | Wilmington Subdivision | 34.59745 | -78.75901 | 57 |
| Norfolk Southern | Winston-Salem District | 36.33427 | -80.19944 | 20 |
| Norfolk Southern | WL Line | 35.36564 | -76.92852 | 10 |
| Yadkin Valley Railroad | Operations | 36.23551 | -80.32055 | 25 |
| Aberdeen and Rockfish Railroad | Operations | 35.05943 | -79.18572 | 17 |
| Aberdeen Carolina and Western Railway | Operations | 35.21461 | -79.62144 | 70 |
| Alexander Railroad | Operations | 35.84797 | -81.03604 | 12 |
| Blue Ridge Southern Railroad | Operations | 35.53009 | -82.8323 | 26 |
| Caldwell County Railroad | Operations | 35.82247 | -81.44066 | 10 |
| Carolina Coastal Railway | Operations | 35.67109 | -77.8856 | 42 |
| High Point Thomasville and Denton Railroad | Operations | 36.0826 | -80.22667 | 7 |
| Laurinburg and Southern Railroad | Operations | 34.69543 | -79.41967 | 7 |
| Atlantic Railways | Operations | 34.36181 | -81.08537 | 5 |
| Amtrak | Raleigh | 35.78023 | -78.64454 | 2 |
| Great Smoky Mountains Railroad | Operations | 35.42324 | -83.44778 | 10 |
| New Hope Valley Railway | Operations | 35.65736 | -78.96583 | 10 |
| North Carolina Transportation Museum | Operations | 35.68858 | -80.43478 | 5 |
| North Carolina and Virginia Railroad | Operations | 35.56691 | -79.59902 | 225 |
| Wilmington Terminal | Operations | 35.56691 | -79.59902 | 225 |
| Charlotte Western Railroad | Operations | 35.56691 | -79.59902 | 225 |
| Raleigh and Fayetteville Railroad (owned by R.J. Corman Railroad) | Operations | 35.39933 | -78.81585 | 50 |
